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[1]包永芬,孙剑刚,袁玉林*.昆虫抗菌肽的诱导及其对荷瘤裸鼠抗瘤作用研究[J].武汉工程大学学报,2010,(12):39-42.[doi:10.3969/j.issn.16742869.2010.12.010]
　BAO Yong fen,SUN Jian gang,YUAN Yu lin.Insectival antibacterial peptides antitumor activity,nude mice bearing, human cervical cancer cell line SiHa[J].Journal of Wuhan Institute of Technology,2010,(12):39-42.[doi:10.3969/j.issn.16742869.2010.12.010]
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2010年12期
页码:
39-42
栏目:
化学与化学工程
出版日期:
2010-12-31

文章信息/Info

Title:
Insectival antibacterial peptides antitumor activity,
nude mice bearing, human cervical cancer cell line SiHa
文章编号:
16742869(2010)12003904
作者:
包永芬1; 2; 孙剑刚2; 袁玉林1*
1.武汉大学基础医学院解剖学教研室，湖北 武汉 430071；
2.咸宁学院基础医学院医学微生物学教研室,湖北 咸宁 437100
Author(s):
BAO Yongfen1; 2;  SUN Jiangang2; YUAN Yulin1
1.Department of Anatonly, Basic Medial College, Wuhan University, Wuhan 430071, China;
2.Department of Medical Microbiology, Xianning University Basic Medical School, Xianning 437100, China
关键词:
昆虫抗菌肽; 裸小鼠; 宫颈癌; SiHa细胞株
Keywords:
insectival antibacterial peptides;  Nude mice;  human cervical cancer;  SiHa
分类号:
TQ465.6
DOI:
10.3969/j.issn.16742869.2010.12.010
文献标志码:
A
摘要:
研究了脂多糖（lipopolysaccharide,LPS）诱导草地贪夜蛾卵细胞系SF9产生抗菌肽的过程.以三氯醋酸提取抗菌肽,抑菌圈法测定抗菌活性,结果显示形成了明显的抑菌圈;体外实验证实,提取的抗菌活性物质能显著抑制人宫颈癌细胞SiHa的生长;体内实验利用已建立的荷人宫颈癌细胞SiHa裸鼠模型,通过局部注射途径进行治疗干预实验,结果显示治疗组与对照组比较肿瘤明显缩小,有显著差异(P<0.01).研究结果表明昆虫抗菌肽具有较明显的抗瘤作用.
Abstract:
Spodoptera frugiperda egg cell line(SF9) were induced with LPS.The insectival antibacterial peptides were recovered by trichloroacetic acid precipitation method,and the antibacterial activity was determined using the zone inhibition assay.They generated obvious inhibition zone.In vitro, antibacterial peptides can inhibit the proliferation of human cervical cancer cell line SiHa.An in vivo analysis in nude mice with tumor xenografts was established to conduct interference treatment.Antibacterial peptides were injected intratumorally.The treatment groups showed marked difference from the control group (P<0.01).Overall, these results indicated that the insectival antibacterial peptides were produced and possesses the potential to be a novel therapeutic agent for treating cervical cancer.

参考文献/References:

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张瑞收稿日期：20100829
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