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[1]胡国元,胡坚,李超影,等.壳寡糖对烟草赤星病病原菌的抑制作用[J].武汉工程大学学报,2012,(12):4-7.[doi:103969/jissn16742869201212002]
　HU Guo yuan,HU Jian,LI Chao ying,et al.Inhibition activites of oligochitosan against alternaria alternate of tobacco[J].Journal of Wuhan Institute of Technology,2012,(12):4-7.[doi:103969/jissn16742869201212002]
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壳寡糖对烟草赤星病病原菌的抑制作用(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年12期
页码:
4-7
栏目:
化学与化学工程
出版日期:
2013-01-11

文章信息/Info

Title:
Inhibition activites of oligochitosan against alternaria alternate of tobacco
文章编号:
16742869(2012)12000404
作者:
胡国元1; 胡坚1; 李超影1; 杨春雷2; 袁军1; 余君2; 杨锦鹏2
1. 武汉工程大学化工与制药学院，绿色化工过程教育部重点实验室，湖北省新型反应器与
绿色化学工艺重点实验室，湖北 武汉 430074；2. 湖北省烟草科研所，湖北 武汉 430030
Author(s):
HU Guoyuan1;  HU Jian1;  LI Chaoying1;  YANG Chunlei2; YUAN Jun1; YU Jun2; YANG Jinpeng2
1. Hubei Key Lab of Novel Reactor & Green Chemical Technology，Key Laboratory for Green Chemical
Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of
Technology, Wuhan 430074, China；2. Tobacco Research Institute of Hubei Province, Wuhan 430030, China
关键词:
壳寡糖; 烟草赤星病; 链格孢菌; 抑制作用
Keywords:
oligochitosan;  tobacco brown spot;  Alternaria alternata;  inhibiting activity
分类号:
Q939.95
DOI:
103969/jissn16742869201212002
文献标志码:
A
摘要:
探讨了壳寡糖对烟草赤星病病原菌（链格孢菌）菌丝生长、孢子萌发、产孢量的影响，并研究了温度和pH值对壳寡糖抑菌作用的影响.结果表明：浓度为15 g/L的壳寡糖在48 h对烟草赤星病病原菌菌丝生长的抑制率为94.74%.壳寡糖抑制病原菌孢子萌发能力强于抑制病原菌菌丝生长，浓度为2.5 g/L的壳寡糖对病原菌孢子萌发抑制率达到100%.质量浓度为10 g/L的壳寡糖在144 h对链格孢菌产孢抑制率为69.7%.壳寡糖的抑菌效果具有良好的热稳定性，pH值对抑菌效果影响较大，质量浓度为15 g/L的壳寡糖在48 h、培养基pH为4时对菌丝生长抑制率达到100%，培养基pH为7时抑制率为62.26%.
Abstract:
The effects of oligochitosan on mycelial growth, spore germination and sporulation of Alternaria alternate of tobacco were explored, and the effects of temperature and pH values to the antibacterial effect of oligochitosan were also studied. The results show that when the concentration of oligochitosan is 15 g/L, the inhibition rate of mycelial growth to mycelial growth of A. alternate is 94.74% in 48 h, and when the concentration of oligochitosan is only 2.5 g/L, the inhibition rate to spore germination of A. alternate is 100%. When the concentration of oligochitosan is 10 g/L, the inhibition rate of sporulation of A. alternate is 69.7% in 144 h. The antibacterial effect of oligochitosan has a good thermal stability. The pH values show great influence to the antibacterial effect of oligochitosan. When the concentration of oligochitosan is 15 g/L, the inhibiton rates of mycelial growth of A. alternate is 100% with the medium pH 4 in 48 h, and 62.26% with the medium pH 7 in 48 h.

参考文献/References:

［1］沈奕.丁几寡糖对烟草黑胫病和赤星病的控制效应及其机制研究\[D\].合肥：安徽农业大学植物保护学院,2004.
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［3］张敏, 何熙璞, 彭好文, 等. 壳寡糖基聚合物对3种病原真菌抑菌作用研究木\[J\]. 中国果树, 2008(3): 3537.
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［5］杨玲玉.壳聚(寡)糖的抑菌规律及对果实采后病害防治作用研究\[D\].青岛：中国海洋大学海洋生物系,2009.
［6］刘晓宇,刘志恒,吕淑霞.壳寡糖对植物病原真菌的抑制作用\[J\].安徽农业科学,2005,33(2):225,282.
［7］卢金清,王琴,何冬黎,等.两种植物源农药对烟草赤星病的药效试验\[J\].湖北农业科学,2011,50(6): 11551157,1161.

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备注/Memo

备注/Memo:
收稿日期：20121104基金项目：武汉工程大学研究生教育创新基金项目（CX201119）作者简介：胡国元（1965），男，湖北红安人，教授，博士．研究方向：生物源农药．
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