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[1]王存文,孟阳,汪铁林,等.三种抑菌剂对蛋白核小球藻生物量和脂质含量的影响[J].武汉工程大学学报,2014,(04):1-6.[doi:103969/jissn16742869201404001]
　WANG Cun wen,MENG Yang,WANG Tie ling,et al.Effect of three bacteriostats on biomass and lipid content of chlorella pyrenoidosa[J].Journal of Wuhan Institute of Technology,2014,(04):1-6.[doi:103969/jissn16742869201404001]
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三种抑菌剂对蛋白核小球藻生物量和脂质含量的影响(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2014年04期
页码:
1-6
栏目:
化学与化学工程
出版日期:
2014-04-30

文章信息/Info

Title:
Effect of three bacteriostats on biomass and lipid content of chlorella pyrenoidosa
文章编号:
16742869(2014)04000106
作者:
王存文1; 2; 孟阳1; 汪铁林1; 2; 吕仁亮1; 2*; 王成成1; 张艳芳1
1.武汉工程大学化工与制药学院，湖北 武汉 430074；2.绿色化工过程教育部重点实验室（武汉工程大学），湖北 武汉 430074\
Author(s):
WANG Cunwen1; 2; MENG Yang1; WANG Tieling1; 2; L Renliang1; 2*; WANG Chengcheng1; ZHANG Yanfang1
1.School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology,Wuhan 430074,China；2.Key Laboratory for Green Chemical Process of Ministry of Education（Wuhan Institute of Technology），Wuhan 430074,China
关键词:
蛋白核小球藻; 抑菌剂; 孟加拉红; 硫酸链霉素; 制霉菌素
Keywords:
chlorella pyrenoidosa; bacteriostatic agent; rose bengal; streptomycin sulfate; nystatin
分类号:
Q819
DOI:
103969/jissn16742869201404001
文献标志码:
A
摘要:
为寻找能够抑制或消灭产油微藻非共生菌且对产油微藻培养有促进作用的抑菌剂，推动富油微藻生产生物柴油的进程，研究了孟加拉红、硫酸链霉素和制霉菌素3种抑菌剂单独和组合分别对蛋白核小球藻生长的影响，并利用超临界二氧化碳萃取法和索氏提取法测定了藻类细胞中的脂质含量的变化.结果表明，制霉菌素会抑制蛋白核小球藻的生长；孟加拉红和硫酸链霉素对蛋白核小球藻的生长表现出积极的促进作用，藻细胞密度的最大值分别为4.216×107 cells/mL和3.512×107 cells/mL，高于不加任何抑菌剂的对照组3.325×107 cells/mL，而对油脂含量几乎没有影响.孟加拉红和硫酸链霉素的混合抑菌剂对蛋白核小球藻的生长起抑制作用.
Abstract:
To find antibacterial agents for suppression or elimination of nonsymbiotic bacteria and promotion of oilrich microalgae cultivation,and then promote biodiesel production process from microalgae,the effects of rose bengal,nystatin and streptomycin sulphate on the growth of chlorella pyrenoidosa were investigated.The lipid contents in algae cells were measured using supercritical CO2 extraction and soxhlet extraction.The results indicate that rose bengal and streptomycin sulphate have almost no effect on lipid content; however,they have a positive effect on stimulating the growth of Chlorella pyrenoidosa,and the maximum values of algae density are 4.216×107 cell/mL and 3.512×107 cell/mL respectively,which are higher than that of control group (3.325×107 cell/mL)without antibiotic.The mixtures of rose bengal and streptomycin sulphate show negative effects on microalgae growth.

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备注/Memo

备注/Memo:
收稿日期：20140319作者简介：王存文（1964），男，湖北鄂州人，教授，博士，博士研究生导师．研究方向：能源化工、化学工程.*通信联系人
更新日期/Last Update: 2014-05-15