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[1]张凯,李茂凡,胡国元*,等.茯苓菌核多糖分离技术研究[J].武汉工程大学学报,2011,(01):25-27.[doi:10.3969/j.issn.16742869.2011.01.007]
　ZHANG Kai,LI Mao fan,HU Guo yuan,et al.Study on isolation technology of polysaccharide of Poria cocos sclerotium[J].Journal of Wuhan Institute of Technology,2011,(01):25-27.[doi:10.3969/j.issn.16742869.2011.01.007]
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茯苓菌核多糖分离技术研究
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2011年01期
页码:
25-27
栏目:
化学与化学工程
出版日期:
2011-01-30

文章信息/Info

Title:
Study on isolation technology of polysaccharide of
Poria cocos sclerotium
文章编号:
16742869(2011)01002503
作者:
张凯1; 李茂凡1; 胡国元1*; 严张微1; 李友国2
1.武汉工程大学化工与制药学院 绿色化工过程省部共建教育部重点实验室,
湖北省新型反应器与绿色工艺重点实验室,湖北 武汉 430074;
2.华中农业大学农业微生物学国家重点实验室,湖北 武汉 430070
Author(s):
ZHANG Kai1;  LI Maofan1;  HU Guoyuan1;  YAN Zhangwei1;  YANG Yang1; LI Weiwei1;  HU Jing;  LI Youguo2
1.Hubei Novel Reactor & Green Chemical Technology Key Laboratory,
Key Laboratory for Green Chemical Process of Ministry of Education,
School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430074, China;
2.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
关键词:
茯苓; 菌核多糖; 热水浸提法; 微波法; 酶法
Keywords:
Poria cocos;  sclerotium polysaccharide;  hot water extraction method;  microwave method;  enzyme method
分类号:
TQ028
DOI:
10.3969/j.issn.16742869.2011.01.007
文献标志码:
A
摘要:
采用单因素试验、正交试验对热水浸提法、微波法、酶法分离茯苓菌核多糖的工艺进行探讨.结果表明: 热水浸提法的最佳工艺条件为:料水比130 (g/mL),浸提温度70 ℃,浸提时间2 h,浸提2次; 微波法最佳提取工艺条件为:料水比130 (g/mL),功率296 W,时间4 min;酶法最佳提取工艺条件为:木瓜蛋白酶0.5％,初始pH 6,温度60 ℃,时间2 h.酶法的多糖提取率最高.采用集成提取工艺的茯苓多糖提取率大幅度提高,酶法与微波组合法的多糖提取率最高.考虑到多糖得率、提取时间、提取成本等因素的影响,最优的集成提取方式为酶法与微波组合法.
Abstract:
Isolation technology of polysaccharide of Poria cocos sclerotium were carried out with hot water extraction method,microwave method and enzyme method through single factor tests and orthogonal tests. The results showed that the optimal conditions of hot water extraction method were extraction at 70 ℃ for 2 h with a ratio of material to water of 130 (g/mL), extraction times 2. And the optimal conditions of microwave method were extraction at 296 W for 4 minutes with a ratio of material to water of 130 (g/mL). And the optimal conditions of enzyme method were that papain, initial pH,time and temperature were 0.5%, 6,2 h and 60 ℃,respectively.The extracting rate of pachyman of enzyme method was the highest. The extracting rates of pachyman of integrated extraction technology were dramatically raised. The extracting rate of pachyman of combined microwave extraction method with enzyme method was the highest. In view of effects of polysaccharide yield, time and costs et al. factors, the optimal integrated extraction technology to was combine enzyme method with microwave method.

参考文献/References:

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相似文献/References:

[1]胡国元,游慧珍,董兰兰,等.茯苓菌丝体液体培养条件研究[J].武汉工程大学学报,2010,(07):1.[doi:10.3969/j.issn.16742869.2010.07.001]
　HU Guo yuan,YOU Hui zhen,DONG Lan lan,et al.Study on mycelia liquid culture of Poria cocos[J].Journal of Wuhan Institute of Technology,2010,(01):1.[doi:10.3969/j.issn.16742869.2010.07.001]

备注/Memo

备注/Memo:
收稿日期：20101003基金项目:湖北省新型反应器与绿色工艺重点实验室开放基金（RGCT200901）作者简介:张凯（1985）,男,湖北武汉人,硕士研究生.研究方向:应用化学.指导老师:胡国元,教授,博士.研究方向:食、药用真菌代谢产物.
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