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[1]余森艳,谢敏,王婷婷.氢氧化钠预处理工艺对花生壳酶解制备还原糖的影响[J].武汉工程大学学报,2012,(8):39-41.[doi:103969/jissn16742869201208010]
　YU Sen yan,XIE Min,WANG Ting ting.Effect of sodium hydroxide pretreatment of peanut shell on reducing sugar by enzymolysis[J].Journal of Wuhan Institute of Technology,2012,(8):39-41.[doi:103969/jissn16742869201208010]
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氢氧化钠预处理工艺对花生壳酶解制备还原糖的影响
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2012年8期
页码:
39-41
栏目:
化学与化学工程
出版日期:
2012-09-10

文章信息/Info

Title:
Effect of sodium hydroxide pretreatment of peanut shell on reducing
sugar by enzymolysis
文章编号:
16742869(2012)08003903
作者:
余森艳; 谢敏; 王婷婷
湖北工程学院新技术学院生物化学系，湖北 孝感 432000
Author(s):
YU Senyan;  XIE Min;  WANG Tingting
Department of Biochemistry, College of Technology, Hubei Engineering University, Xiaogan 432000, China
关键词:
固体废渣; 预处理; 生物降解; 葡萄糖
Keywords:
solid waste slag;  pretreatment;  biodegradation;  glucose
分类号:
TQ353.4+2
DOI:
103969/jissn16742869201208010
文献标志码:
A
摘要:
针对花生壳直接酶解制备还原糖得率低的问题，提出了氢氧化钠（NaOH）预处理再进行酶解的方法.该方法通过对NaOH浓度、预处理温度、预处理时间进行单因素试验，然后在单因素试验的基础上确定正交试验工艺参数范围，以酶解后还原糖得率为指标，得出NaOH预处理花生壳的优化工艺条件.结果表明：在试验范围内固液比1∶10，预处理工艺条件为NaOH质数分数2%，温度70 ℃，预处理时间6 h，纤维素酶水解后还原糖得率达到了72.5%，较未处理前提高了53.9%.
Abstract:
Pretreatment of peanut shell by sodium hydroxide and then enzymolysis was proposed to solve the low yield of reducing sugar. Through singlefactor trial of sodium hydroxide concentration, pretreatment temperature and time and orthogonal design, the optimal pretreatment technology was determined with the hydrolysis of reducing sugar as index. The results show that the peanut shell and 2% of sodium hydroxide in the case of 10∶1 at 70 ℃ processing 6 hours is the best pretreatment technology, and the reducing sugar content by enzymolysis is up to 72.5%, which increases by 53.9% compared with that before pretreatment.

参考文献/References:

［1］周泉城,黄景荣,李全宏. 花生壳茎叶等综合利用研究进展\[J\]. 中国粮油学报, 2009,24(10):140144.
［2］李春光, 董令叶, 吉洋洋, 等. 花生壳纤维素提取及半纤维素与木质素脱除工艺探讨\[J\]. 中国农学通报, 2010, 26(22): 350354.
［3］鲁杰,石淑兰,邢效功,等. NaOH预处理对植物纤维素酶解特性的影响\[J\]. 纤维素科学与技术: 2004,12(1): 16.
［4］陈毓荃. 生物化学实验方法和技术\[M\]. 北京: 科学出版社，2002.
［5］唐楚颖, 马春燕, 陆豫, 等. 绿色木霉降解木薯杆产糖及其工艺优化研究\[J\]. 安徽农业科学, 2011, 39(31): 1931019312.
［6］MZuhairs. The effect of erystallinity of cellulose on the rate of reducing sugars production by heterogeneous enzymatic hydrolysis \[J\]. Bioresource Technology, 2008, 99: 40784085.

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备注/Memo

备注/Memo:
收稿日期：20120423作者简介：余森艳（1977），女，湖北应城人,讲师，硕士.研究方向：生物工程技术及发酵产品.
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