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[1]徐春放,贺志丽.浊点萃取分光光度法测头发中锌的含量[J].武汉工程大学学报,2013,(10):17-22.[doi:103969/jissn16742869201310004]
　XU Chun\|fang,HE Zhi\|li.Measuring content of zinc in hair by cloud point extraction spectrophotometry[J].Journal of Wuhan Institute of Technology,2013,(10):17-22.[doi:103969/jissn16742869201310004]
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浊点萃取分光光度法测头发中锌的含量(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2013年10期
页码:
17-22
栏目:
化学与化学工程
出版日期:
2013-11-10

文章信息/Info

Title:
Measuring content of zinc in hair by cloud point extraction spectrophotometry
文章编号:
16742869(2013)10001706
作者:
徐春放; 贺志丽
大庆师范学院化学化工学院，黑龙江 大庆163712
Author(s):
XU Chun\|fang;  HE Zhi\|li
Department of Chemistry & Chemical Engineering, Daqing Normal University, Daqing 163712, China
关键词:
浊点萃取; 分光光度法; 锌; 曲拉通X\|114
Keywords:
cloud point extraction;  spectrophotometric method;  Zinc;  triton X\|114
分类号:
O657.32
DOI:
103969/jissn16742869201310004
文献标志码:
A
摘要:
针对传统萃取分光光度法测定头发中的锌含量，需要使用苯、四氯化碳等有机毒性试剂萃取锌\|双硫腙疏水配合物，存在污染环境和操作费时之弊端，采用浊点萃取法代替有机毒性试剂萃取锌\|双硫腙红色疏水配合物，用分光光度法测定人发中的锌含量.选择湿法消化处理头发试样，选取非离子表面活性剂曲拉通X\|114和硫酸铵作为浊点萃取体系，对锌\|双硫腙红色疏水配合物进行浊点萃取；实验测定了浊点萃取物的最大吸收波长、萃取剂的浓度、萃取剂的测定、硫酸铵的用量及显色体系的pH和显色剂的用量对吸光度的影响，并进行回收率实验.实验结果表明浊点萃取的配合物最大吸收波长为527 nm、质量分数2%的萃取剂为0.3～1.0 mL、硫酸铵1.2 g、pH为4.5、显色剂的用量为0.2～0.8 mL，锌含量的线性范围为0.2～1.2 μg/mL，线性回归方程为A=0.435 9 c(μg/mL)+0.014 8, 相关系数r=0.997 3，表观摩尔吸光系数为5.894×10 000 L/（mol·cm），测定结果的相对偏差为2.4%，回收率为98.76%～102.2%.用该法测定头发中的微量锌，结果令人满意，对食品中微量锌的测定具有一定应用价值.
Abstract:
For the drawbacks that measuring the zinc content in hair with traditional extraction spectrophotometric method need to use benzene, carbon tetrachloride and other organic toxic reagent to extract Zn\|dithizone red hydrophobic complexes leads to a result of pollution of the environment and time\|consuming extraction operation, cloud point extraction method was used to extract Zn\|dithizone red hydrophobic complexes in stead of organic toxic reagent and the content of zinc in hair is measured with spectrophotometric method. Selecting wet digestion processing hair samples and non\|ionic surfactant Triton X\|114 and ammonium sulfate as the cloud point extraction system,Zn\|dithizone red hydrophobic complexes are carried on the cloud point extract.The experiment determines the influences of the maximum absorption wavelength of the complexes, the density of the extracting agent, the determination extracting agent, the amount of ammonium sulfate,the pH of colored system,the amount of chromogenic agent on absorbance, and carries on the returns\|ratio experiment. Experimental results show that the maximum absorption wavelength of the complexes is 527 nm, the extractant of 2% is 0.3~1.0 mL,the amount of ammonium sulfate is 1.2 g,the determination of pH is 4.5, the dosage range of chromogenic reagent is 0.2-0.8 mL,the proportional range of zinc content is 0.2-1.2 μg/mL, the equation of linear regression A=0.435 9 c(μg/mL) + 0.014 8, the coefficient of correlation r=0.997 3,the apparent molar absorptivity is 5.894×10 000 L/（mol ·cm） and relative deviation of the test result is 2.4%. Determining the micro zinc in the hair in this way can get a satisfying result: coefficient of recovery is 98.76%-102.2%, which has a certain application value for de terminating micro zinc in food.

参考文献/References:

\[1\]池华，张华，郝子峰. 脑梗死患者头发中锌\|铜\|铬\|镍含量测定\[J\]. 黑龙江医学，2003，27（2）：131.CHI Hua，ZHANG Hua,HE Zi\|feng. Determination of total contents of Zn, Cu, Cr and Ni in hair of people \[J\]. Hei Long Jiang Medical Journal, 2003, 27(2): 131. (in Chinese)\[2\]江天，姜怡帆，陈明石，等. 人发中锌铬镁元素与代谢综合征相关疾病的研究分析\[J\]. 微量元素与健康研究，2008，25（4）：54\|55.JIANG Tian, JIANG Yi\|fan, CHEN Ming\|shi, et al. The relationship between zinc, chrominu, magnesium in hair and MS diseases \[J\]. Studies of Trace Elements and Health, 2008, 25(5): 54\|55. (in Chinese)\[3\]秦俊法，李增禧，楼蔓藤，等. 头发元素分析的科学意义及医学应用价值\[J\]. 广东微量元素科学，2005，12 （5）：21\|60.QIN Jun\|fa, LI Zeng\|xi, LOU Man\|teng, et al. Scientific value and medical application of hair element analysis［J］. Trace Elements in Guangdong Science, 2005, 12(5): 21\|60. (in Chinese)\[4\]Sbel C E, Neureuther J M, Siemann S. A spectrophotometric method for the determination of zinc, copper, and cobalt ions in metalloproteins using zincon \[J\]. Analytical Biochemistry, 2010, 397(2): 218\|226.\[5\]司文会，朱金坤. 双硫腙萃取光度法测定锌的研究\[J\]. 分析科学学报，2002，18（6）：522.SI Wen\|hui, ZHU Jin\|kun. A study on spectrophotometric determination of zinc with dithizone extraction \[J\]. Journal of Analytical Science, 2002, 18(6): 522. (in Chinese)\[6\]郭虹，王宝旺. 锌试剂\|环己酮分光光度法测定乳制品奶粉中的锌\[J\] 中国卫生检验杂志，2006，16（6）：673\|674.GUO Hong, WANG Bao\|wang. Determination of zinc dairy products in milk powder with zincon\|cyclohexanone spectrophotometry \[J\]. Chinese Journal of Health Laboratory Technology, 2006, 16(6): 673\|674. (in Chinese)\[7\]赵小波，王京平，费正皓，等. 人发中微量锌的PAN\| S分光光度法测定研究\[J\]. 化学世界，2011，52（9）：519\|522. ZHAO Xiao\|bo, WANG Jing\|ping, FEI Zheng\|hao, et al. Spectrometric analysis of microelement zinc in human hairs \[J\]. Chemical World, 2011, 52(9): 519\|522. (in Chinese)\[8\]赵桦萍，靳凤丹，于岩. 人发中锌的分光光度法测定\[J\]. 齐齐哈尔大学学报，2012，28（6）：39\|40.ZHAO Ye\|ping, JIN Feng\|dan, YU Yan. Determination of zinc in hair by spectrophotometry \[J\]. Journal of Qiqihar University, 2012, 28(6): 39\|40. (in Chinese) \[9\]Gao Y, Wu P, Li W, et al. Simultaneous and selective preconcentration of trace Cu and Ag by one\|step displacement cloud point extraction for FAAS determination \[J\], Talanta, 2010, 81 (1\|2): 586\|590.\[10\]Sang H, Liang P, Du D. Determination of trace aluminum in biological and water samples by cloud point extraction preconcentration and graphite furnace atomic absorption spectrometry detection \[J\]. Journal of Hazardous Materials, 2008, 154 (1/2/3): 1127\|1132.\[11\]Silva M， Cerutti E， Martinez L. Coupling cloud point extraction to instrumental detection systems for metal analysis \[J\]. Microchimica Acta, 2006,155(3/4): 349\|364.\[12\]董树国. 浊点萃取\|分光光度法测定茶叶中的痕量锌\[J\]. 光谱实验室，2011，28（6）：3124\|3127.DONG Shu\|guo. Determination of trace zinc in tea by spectrophotometry with cloud point extraction \[J\]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(6):3124\|3127. (In Chinese)\[13\]李印龙，公维磊，宋爱芹，等. 不同消化法测定头发铜铁锌含量效果比较\[J\]. 济宁医学院学报，2010，33（1）：57\|58.LI Yin\|long, GONG Wei\|lei, SONG Ai\|qin, et al. Study on different digestion methods for determination of Cu, Fe and Zn content in hair \[J\]. Journal of Jining Medical University, 2010, 33(1): 57\|58. (in Chinese)\[14\]高琳，陈圣洁，石红，等. 非离子表面活性剂在浊点萃取及光度分析中的应用研究\[J\]. 胶体与聚合物，2012，30（3）：130\|132.GAO Lin, CHEN Sheng\|jie, SHI Hong, et al. Application of nonionic surfactant in cloud point extraction and spectrophotometry \[J\]. Chinese Journal of Colloid ＆Polymer, 2012, 30(3): 130\|132. (in Chinese)\[15\]孟庆华，孙志成，王鸣华，等. 利用紫外\|可见光谱仪测定表面活性剂浊点的新方法\[J\]. 实验室研究与探索，2006，25（2）：163\|165.MENG Qing\|hua, SUN Zhi\|cheng, WANG Ming\|hua, et al. A new determination method of surfactant’s cloud point by UV\|vis spectrometer \[J\]. Research and Exploration in Laboratory, 2006, 25(2): 163\|165. (in Chinese)\[16\]黄晖，杨大雨. 浊点萃取分光光度法测定水样中的痕量锌（II）\[J\]. 广东微量元素科学，2008，15（6）：61\|65.HUANG Hui, YANG Da\|yu. Determination of trace amount of zinc in river water by spectrophotometry after cloud point extraction \[J\]. Trace Elements in Guangdong Science, 2008, 15(6): 61\|65. (in Chinese)\[17\]黄炎，秦炜，丁昱文，等. 非离子表面活性剂TX\|114浊点萃取D2EHPA的规律\[J\]. 化工学报，2008，59（2）：393\|397.HUANG Yan, QIN Wei, DING Yu\|wen, et al. Cloud point extraction of di (2\|ethylhexyl) phosphoric acid utilizing non\|ionic surfactant TX14 \[J\]. Journal of Chemical Industry and Engineering (China), 2008, 59(2): 393\|397. (in Chinese)\[18\]张桂平，秦炜，戴猷元. 浊点萃取及应用\[J\]. 中国科技论文在线，2007，2（12）：1\|4.ZHANG Gui\|ping, QIN Wei, DAI You\|yuan. The cloud point extraction and its applications \[J\].Sciencepaper online（http://www.paper.edu.cn）, 2007, 2(12): 1\|4. (in Chinese）

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备注/Memo

备注/Memo:
收稿日期：20130315作者简介：徐春放（1963\|），女，吉林长岭人，教授，硕士.研究方向：分析化学理论、实验教学和研究.
更新日期/Last Update: 2013-11-11