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[1]王 璇,张 旗*,方 成,等.Bola表面活性剂与牛血清蛋白相互作用的光谱研究[J].武汉工程大学学报,2017,39(02):120-126.[doi:10. 3969/j. issn. 1674?2869. 2017. 02. 004]
 WANG Xuan,ZHANG Qi*,FANG Cheng,et al.Spectroscopic Studies on Interaction of Bovine Serum Albumin with Bola Surfactant[J].Journal of Wuhan Institute of Technology,2017,39(02):120-126.[doi:10. 3969/j. issn. 1674?2869. 2017. 02. 004]
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Bola表面活性剂与牛血清蛋白相互作用的光谱研究(/HTML)
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
39
期数:
2017年02期
页码:
120-126
栏目:
化学与化学工程
出版日期:
2017-05-04

文章信息/Info

Title:
Spectroscopic Studies on Interaction of Bovine Serum Albumin with Bola Surfactant
作者:
王 璇张 旗*方 成周泽坤刘治田
1. 武汉工程大学材料科学与工程学院,湖北 武汉 430205;2. 等离子体化学与新材料湖北省重点实验室(武汉工程大学),湖北 武汉 430205
Author(s):
WANG Xuan ZHANG Qi* FANG Cheng ZHOU Zekun LIU Zhitian
1. School of Materials Science and Engineering,Wuhan Institute of Technology,Wuhan 430205,China;2. Hubei Key Laboratory of Plasma Chemical and Advanced Materials (Wuhan Institute of Technology),Wuhan 430205,China
关键词:
Bola表面活性剂牛血清蛋白荧光光谱圆二色谱
Keywords:
Bola surfactant BSA fluorescence spectrum circular dichroism spectrum
分类号:
O657
DOI:
10. 3969/j. issn. 1674?2869. 2017. 02. 004
文献标志码:
A
摘要:
采用荧光光谱法、紫外吸收光谱法和圆二色谱法研究了在Na2HPO3-NaH2PO3缓冲溶液(pH=7.4,298 K)中三种不同头基的Bola表面活性剂Bola(Me)、Bola(Et)、Bola(Pr)与牛血清蛋白(BSA)的相互作用,考察了表面活性剂浓度、结构对相互作用的影响,初步阐明了Bola表面活性剂与BSA的结合机理. 结果表明:三种Bola表面活性剂均对BSA的荧光强度有静态猝灭作用,并导致其最大发射波长蓝移;表面活性剂的加入主要影响BSA的色氨酸(Trp)残基;表面活性剂头基的烷基链越长,Stern-Volmer?猝灭常数越大,表明具有较长头基烷基链的表面活性剂与BSA具有更强的相互作用.
Abstract:
Interaction between a Bola series of surfactants Bola(Me),Bola(Et)and Bola(Pr) with different headgroups and bovine serum albumin (BSA) in Na2HPO3-NaH2PO3 buffer solution (pH=7.4,298 K) were studied by using fluorescence spectroscopy, UV absorption spectroscopy and circular dichroism spectroscopy in this paper. Effects of the surfactants structure and concentration on the interaction were examined, and the binding mechanism of Bola surfactants and BSA were elucidated.The experimental results showed that all the Bola surfactants had a static quenching effect on the fluorescence intensity of bovine serum albumin (BSA) and resulted in a blue shift of the maximum emission wavelength. The addition of surfactants mainly affected the tryptophan residues of BSA. The longer alkyl chain the surfactant has, the larger the Stern-Volmer quenching constant is, indicating that the surfactant with longer head alkyl chain has stronger interaction with BSA.

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更新日期/Last Update: 2017-04-25